Typing of human and bovine Staphylococcus aureus by RAPD-PCR and ribotyping-PCR
نویسندگان
چکیده
منابع مشابه
Pcr-ribotyping of Bovine and Human Methicillin- Resistant Staphylococcus Aureus
The present study aimed at the assessment of genetic relatedness among the methicillin-resistant Staphylococcus aureus (MRSA) isolates of bovine and human origin. A set of twenty-four MRSA isolates obtained from cattle (11), buffaloes (10) and their handlers (3) were subjected to PCR-ribotyping using primers specific for 16S-23S ribosomal spacer region. Polymorphism was observed with 23 ribotyp...
متن کاملPCR-based ribotyping of Staphylococcus aureus.
Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for ...
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The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the ga...
متن کاملRAPD-PCR and Drug Resistance Pattern of Staphylococcus aureus Isolates Recovered from Companion and Wild Birds
BACKGROUND: Staphylococcus aureus is a highly versatile pathogen of a large number of domestic ani- mals, including avian species. There is limited information about S. aureus isolated from companion and wild birds in Iran. OBJECTIVES: The aim of this study was to determine drug resistance and random-amplified polymorphic DNA-PCR (RAPD-P...
متن کاملCharacterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats
Pasteurella multocida is considered to be an important cause of ovine pneumonia and results causes considerable economic losses in Iran. Ribotyping PCR (Ribo-PCR) was used for investigating the diversity of ovine and caprine P.multocida. A total of 120 swab tonsil and nasal samples were obtained from sheep and goats. They were analyzed by biochemical tests and Ribo-PCR 16S-23S ribosomal RNA gen...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2002
ISSN: 0266-8254,1365-2673
DOI: 10.1046/j.1472-765x.2002.01127.x